DMPQ: . What is Polymerase Chain reaction? What are the materials required for Polymerase chain reaction?

:  The polymerase chain reaction is a technique for cloning a particular piece or amplifying a single or few copies of a piece of DNA, generating millions or more copies of that particular DNA sequence. One can make virtually unlimited copies of a single DNA molecule even though it may initially be present in a mixture containing many different DNA molecules.

 

MATERIAL REQUIRED

A basic PCR requires the following components:

  • DNAtemplatethat contains the DNA region called target to be amplified.
  • Two primerscomplementary to the DNA regions at the 5? or 3? end of DNA.
  • The enzyme, Taq polymeraseor another DNA polymerase with a temperature optimum at around 70°C.
  • Deoxynucleoside triphosphates (dNTPs)that are building blocks for DNA synthesis, e.g. dATP, dGTP, dCTP and dTTP.
  • Buffer solution, providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.
  • Divalent cations, magnesium or manganese ions; generally Mg2+ is used but Mn2+ can be utilized for PCR-mediated DNA mutagenesis.
  • Monovalent cationpotassium ions.

 

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